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anti chrebp antibody  (Novus Biologicals)


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    Structured Review

    Novus Biologicals anti chrebp antibody
    Experimental validation of MLXIPL upregulation and metabolic network consistency in diabetic models. (A–C) Assessment of <t>ChREBP</t> expression in kidney tissues from db/m and db/db mice. Representative Western blots (A) and densitometric quantification (B) of ChREBP protein levels. For animal Western blot analyses, densitometric quantification was performed using all 12 mice per group. Full-length immunoblot images are provided in <xref ref-type=Supplementary Figure 4 . (C) Relative mRNA expression of Mlxipl . (D–F) Assessment of ChREBP expression in primary proximal tubular epithelial cells (PTECs) under normal glucose (NC) and high glucose (HG) conditions. Representative Western blots (D) , protein quantification (E) , and mRNA levels (F) . (G) Representative immunohistochemistry (IHC) staining of ChREBP in mouse kidney sections. Scale bar: 100 μm. (H) Representative immunofluorescence (IF) staining of ChREBP (green) in PTECs; nuclei were counterstained with DAPI (blue). Scale bar: 50 μm. (I) Relative mRNA expression of MLXIPL -interacting metabolic genes in mouse kidneys. (J) Pearson correlation analysis between Mlxipl expression and downstream metabolic targets in kidney tissues.Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. " width="250" height="auto" />
    Anti Chrebp Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+chrebp+antibody/CHREBP+Antibody+-+BSA+Free/pmc13125001-141-9-11
    Average 95 stars, based on 115 article reviews
    anti chrebp antibody - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Integrated causal inference, kidney transcriptomics, and experimental validation identify ChREBP ( MLXIPL ) as a driver of maladaptive metabolic remodeling in diabetic kidney disease"

    Article Title: Integrated causal inference, kidney transcriptomics, and experimental validation identify ChREBP ( MLXIPL ) as a driver of maladaptive metabolic remodeling in diabetic kidney disease

    Journal: Frontiers in Endocrinology

    doi: 10.3389/fendo.2026.1809567

    Experimental validation of MLXIPL upregulation and metabolic network consistency in diabetic models. (A–C) Assessment of ChREBP expression in kidney tissues from db/m and db/db mice. Representative Western blots (A) and densitometric quantification (B) of ChREBP protein levels. For animal Western blot analyses, densitometric quantification was performed using all 12 mice per group. Full-length immunoblot images are provided in <xref ref-type=Supplementary Figure 4 . (C) Relative mRNA expression of Mlxipl . (D–F) Assessment of ChREBP expression in primary proximal tubular epithelial cells (PTECs) under normal glucose (NC) and high glucose (HG) conditions. Representative Western blots (D) , protein quantification (E) , and mRNA levels (F) . (G) Representative immunohistochemistry (IHC) staining of ChREBP in mouse kidney sections. Scale bar: 100 μm. (H) Representative immunofluorescence (IF) staining of ChREBP (green) in PTECs; nuclei were counterstained with DAPI (blue). Scale bar: 50 μm. (I) Relative mRNA expression of MLXIPL -interacting metabolic genes in mouse kidneys. (J) Pearson correlation analysis between Mlxipl expression and downstream metabolic targets in kidney tissues.Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. " title="... network consistency in diabetic models. (A–C) Assessment of ChREBP expression in kidney tissues from db/m and db/db ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Experimental validation of MLXIPL upregulation and metabolic network consistency in diabetic models. (A–C) Assessment of ChREBP expression in kidney tissues from db/m and db/db mice. Representative Western blots (A) and densitometric quantification (B) of ChREBP protein levels. For animal Western blot analyses, densitometric quantification was performed using all 12 mice per group. Full-length immunoblot images are provided in Supplementary Figure 4 . (C) Relative mRNA expression of Mlxipl . (D–F) Assessment of ChREBP expression in primary proximal tubular epithelial cells (PTECs) under normal glucose (NC) and high glucose (HG) conditions. Representative Western blots (D) , protein quantification (E) , and mRNA levels (F) . (G) Representative immunohistochemistry (IHC) staining of ChREBP in mouse kidney sections. Scale bar: 100 μm. (H) Representative immunofluorescence (IF) staining of ChREBP (green) in PTECs; nuclei were counterstained with DAPI (blue). Scale bar: 50 μm. (I) Relative mRNA expression of MLXIPL -interacting metabolic genes in mouse kidneys. (J) Pearson correlation analysis between Mlxipl expression and downstream metabolic targets in kidney tissues.Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

    Techniques Used: Biomarker Discovery, Expressing, Western Blot, Immunohistochemistry, Immunofluorescence, Staining

    Related Articles

    Incubation:

    Article Title: Integrated causal inference, kidney transcriptomics, and experimental validation identify ChREBP ( MLXIPL ) as a driver of maladaptive metabolic remodeling in diabetic kidney disease
    Article Snippet: Sections were blocked with 1% BSA and incubated with anti-ChREBP antibody (Novus Biologicals, NB400-135; IHC dilution 1:200), followed by appropriate secondary antibodies and chromogenic development. .. For IF, PTECs were incubated with anti-ChREBP antibody (Novus Biologicals, NB400-135; IF dilution 1:50), followed by fluorescent secondary antibodies and DAPI counterstaining. ..

    Article Title: Integrated causal inference, kidney transcriptomics, and experimental validation identify ChREBP ( MLXIPL ) as a driver of maladaptive metabolic remodeling in diabetic kidney disease
    Article Snippet: .. Sections were blocked with 1% BSA and incubated with anti-ChREBP antibody (Novus Biologicals, NB400-135; IHC dilution 1:200), followed by appropriate secondary antibodies and chromogenic development. .. For IF, PTECs were incubated with anti-ChREBP antibody (Novus Biologicals, NB400-135; IF dilution 1:50), followed by fluorescent secondary antibodies and DAPI counterstaining.

    Article Title: Fructose overconsumption impairs hepatic manganese homeostasis and ammonia disposal.
    Article Snippet: .. After sonication, fragmented chromatin was incubated overnight at 4°C with anti-ChREBP antibody (Novus, NB400-135), or isotype IgG (Upstate) as negative control, and followed by incubation with Dynabead-conjugated Protein G (Invitrogen). ..

    Article Title: Fructose overconsumption impairs hepatic manganese homeostasis and ammonia disposal
    Article Snippet: .. After sonication, fragmented chromatin was incubated overnight at 4°C with anti-ChREBP antibody (Novus, NB400-135), or isotype IgG (Upstate) as negative control, and followed by incubation with Dynabead-conjugated Protein G (Invitrogen). ..

    Article Title: Dual regulation of TxNIP by ChREBP and FoxO1 in liver
    Article Snippet: .. Briefly, proteins were incubated with 2 μg of anti-ChREBP antibody (Novus) and placed at 4°C overnight. .. Bound proteins were recovered after addition of 30 μl of Sepharose-labeled protein G (Sigma) for 1 h at 4°C.

    Chromatin Immunoprecipitation:

    Article Title: Effects of ChREBP deficiency on adrenal lipogenesis and steroidogenesis.
    Article Snippet: .. ChIP assay was performed using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (Catalogue no. 9005, Cell Signaling Technology Inc.) and anti-ChREBP antibody (Catalogue no. NB400-135, Novus Biologicals, Centennial, CO) or control IgG according to manufacturer’s protocol. ..

    Magnetic Beads:

    Article Title: Effects of ChREBP deficiency on adrenal lipogenesis and steroidogenesis.
    Article Snippet: .. ChIP assay was performed using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (Catalogue no. 9005, Cell Signaling Technology Inc.) and anti-ChREBP antibody (Catalogue no. NB400-135, Novus Biologicals, Centennial, CO) or control IgG according to manufacturer’s protocol. ..

    Control:

    Article Title: Effects of ChREBP deficiency on adrenal lipogenesis and steroidogenesis.
    Article Snippet: .. ChIP assay was performed using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (Catalogue no. 9005, Cell Signaling Technology Inc.) and anti-ChREBP antibody (Catalogue no. NB400-135, Novus Biologicals, Centennial, CO) or control IgG according to manufacturer’s protocol. ..

    Immunohistochemistry:

    Article Title: Integrated causal inference, kidney transcriptomics, and experimental validation identify ChREBP ( MLXIPL ) as a driver of maladaptive metabolic remodeling in diabetic kidney disease
    Article Snippet: .. Sections were blocked with 1% BSA and incubated with anti-ChREBP antibody (Novus Biologicals, NB400-135; IHC dilution 1:200), followed by appropriate secondary antibodies and chromogenic development. .. For IF, PTECs were incubated with anti-ChREBP antibody (Novus Biologicals, NB400-135; IF dilution 1:50), followed by fluorescent secondary antibodies and DAPI counterstaining.

    Sonication:

    Article Title: Fructose overconsumption impairs hepatic manganese homeostasis and ammonia disposal.
    Article Snippet: .. After sonication, fragmented chromatin was incubated overnight at 4°C with anti-ChREBP antibody (Novus, NB400-135), or isotype IgG (Upstate) as negative control, and followed by incubation with Dynabead-conjugated Protein G (Invitrogen). ..

    Article Title: Fructose overconsumption impairs hepatic manganese homeostasis and ammonia disposal
    Article Snippet: .. After sonication, fragmented chromatin was incubated overnight at 4°C with anti-ChREBP antibody (Novus, NB400-135), or isotype IgG (Upstate) as negative control, and followed by incubation with Dynabead-conjugated Protein G (Invitrogen). ..

    Negative Control:

    Article Title: Fructose overconsumption impairs hepatic manganese homeostasis and ammonia disposal.
    Article Snippet: .. After sonication, fragmented chromatin was incubated overnight at 4°C with anti-ChREBP antibody (Novus, NB400-135), or isotype IgG (Upstate) as negative control, and followed by incubation with Dynabead-conjugated Protein G (Invitrogen). ..

    Article Title: Fructose overconsumption impairs hepatic manganese homeostasis and ammonia disposal
    Article Snippet: .. After sonication, fragmented chromatin was incubated overnight at 4°C with anti-ChREBP antibody (Novus, NB400-135), or isotype IgG (Upstate) as negative control, and followed by incubation with Dynabead-conjugated Protein G (Invitrogen). ..

    Expressing:

    Article Title: 1, 3-dioxolane derivative
    Article Snippet: .. In the SV40MES13-ChoRE-Luc_FLAG-ChREBP cells, anti-FLAG antibody (Merck KGaA)- and anti-ChREBP antibody (Novus Biologicals, LLC)-positive bands were confirmed, and the expression of ChREBP protein derived from pQCXIH FLAG ChREBP was confirmed. ..

    Derivative Assay:

    Article Title: 1, 3-dioxolane derivative
    Article Snippet: .. In the SV40MES13-ChoRE-Luc_FLAG-ChREBP cells, anti-FLAG antibody (Merck KGaA)- and anti-ChREBP antibody (Novus Biologicals, LLC)-positive bands were confirmed, and the expression of ChREBP protein derived from pQCXIH FLAG ChREBP was confirmed. ..



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    Experimental validation of MLXIPL upregulation and metabolic network consistency in diabetic models. (A–C) Assessment of ChREBP expression in kidney tissues from db/m and db/db mice. Representative Western blots (A) and densitometric quantification (B) of ChREBP protein levels. For animal Western blot analyses, densitometric quantification was performed using all 12 mice per group. Full-length immunoblot images are provided in <xref ref-type=Supplementary Figure 4 . (C) Relative mRNA expression of Mlxipl . (D–F) Assessment of ChREBP expression in primary proximal tubular epithelial cells (PTECs) under normal glucose (NC) and high glucose (HG) conditions. Representative Western blots (D) , protein quantification (E) , and mRNA levels (F) . (G) Representative immunohistochemistry (IHC) staining of ChREBP in mouse kidney sections. Scale bar: 100 μm. (H) Representative immunofluorescence (IF) staining of ChREBP (green) in PTECs; nuclei were counterstained with DAPI (blue). Scale bar: 50 μm. (I) Relative mRNA expression of MLXIPL -interacting metabolic genes in mouse kidneys. (J) Pearson correlation analysis between Mlxipl expression and downstream metabolic targets in kidney tissues.Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. " width="100%" height="100%">

    Journal: Frontiers in Endocrinology

    Article Title: Integrated causal inference, kidney transcriptomics, and experimental validation identify ChREBP ( MLXIPL ) as a driver of maladaptive metabolic remodeling in diabetic kidney disease

    doi: 10.3389/fendo.2026.1809567

    Figure Lengend Snippet: Experimental validation of MLXIPL upregulation and metabolic network consistency in diabetic models. (A–C) Assessment of ChREBP expression in kidney tissues from db/m and db/db mice. Representative Western blots (A) and densitometric quantification (B) of ChREBP protein levels. For animal Western blot analyses, densitometric quantification was performed using all 12 mice per group. Full-length immunoblot images are provided in Supplementary Figure 4 . (C) Relative mRNA expression of Mlxipl . (D–F) Assessment of ChREBP expression in primary proximal tubular epithelial cells (PTECs) under normal glucose (NC) and high glucose (HG) conditions. Representative Western blots (D) , protein quantification (E) , and mRNA levels (F) . (G) Representative immunohistochemistry (IHC) staining of ChREBP in mouse kidney sections. Scale bar: 100 μm. (H) Representative immunofluorescence (IF) staining of ChREBP (green) in PTECs; nuclei were counterstained with DAPI (blue). Scale bar: 50 μm. (I) Relative mRNA expression of MLXIPL -interacting metabolic genes in mouse kidneys. (J) Pearson correlation analysis between Mlxipl expression and downstream metabolic targets in kidney tissues.Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

    Article Snippet: Sections were blocked with 1% BSA and incubated with anti-ChREBP antibody (Novus Biologicals, NB400-135; IHC dilution 1:200), followed by appropriate secondary antibodies and chromogenic development.

    Techniques: Biomarker Discovery, Expressing, Western Blot, Immunohistochemistry, Immunofluorescence, Staining